分子機構研究

メンバー

リーダー 研究部長　萬谷 博
研究員 赤阪 啓子、今江 理恵子

キーワード

アルツハイマー病、個体老化、蛋白質分解、糖鎖、糖蛋白質、神経疾患、筋疾患、認知症、老化関連疾患、老化モデルマウス（Klotho）

主な研究

  1. 認知症における糖鎖の解析
  2. 老化モデルマウスを用いた老化機構の解明
  3. 神経筋維持に関連する糖鎖機能の解明

研究紹介

  1. 認知症における糖鎖関連遺伝子の発現変化を網羅的に解析し、どのような遺伝子が関与するかを明らかにします。また、Aβ産生機構と糖鎖の関連を解析し、新たな治療および予防法の開発を目指します。
  2. 老化モデルマウス(Klotho)における責任遺伝子産物の生理機能の解明を目指します。さらに、老化モデルマウスで得られた研究成果の自然老化マウスでの検証および老化関連疾患との関連性を明らかにします。
  3. 神経や筋肉における糖鎖の生理機能や生合成機構を解明し、老化や疾患に伴う変化の実体と病態との関連性を明らかにします。

生命の設計図であるゲノムの遺伝子情報は翻訳されてタンパク質が作られますが、半数以上のタンパク質はこのままではまだ働きが不十分で、そこにリン酸や糖鎖というものがくっついてリン酸化タンパク質や糖タンパク質となり初めて機能的に働きます。これをタンパク質の翻訳後修飾といい、生物のさまざまな機能にかかわっています。本研究グループは細胞どうしの認識や細胞内外の情報伝達において重要な役割を果たしているタンパク質の翻訳後修飾に焦点を当て、老化に伴う機能変化の解明および老化に伴う病態の解明を目指しています。

主要文献

【英文】

  1. Manya, H.: Structure and biosynthesis of O-mannosyl glycans in mammals. Glycoword, Glyco forum, GD-C07E, 2024, (https://www.glycoforum.gr.jp/glycoword/index.html)
  2. Hoshino, S., #Manya, H., Imae, R., Kobayashi, K., Kanagawa, M., #Endo, T.: Endogenous reductase activities for the generation of ribitol-phosphate, a CDP-ribitol precursor, in mammals. J Biochem. mvad115, 2024, in press (#co-coresponding)
  3. Tachida, Y., Iijima, J., Takahashi, K., Suzuki, H., Kizuka, Y., Yamaguchi, Y., Tanaka, K., Nakano, M., Takakura, D., Kawasaki, N., Saito, Y., Manya, H., Endo, T., Kitazume, S.: O-GalNAc glycosylation determines intracellular trafficking of APP and Aβ production. J. Biol. Chem. 299(7), 104905, 2023
  4. Hoshino, S., #Manya, H., Endo, T.: Enzyme assay of ribitol xylosyltransferase 1 (RXYLT1). Glycoscience Protocols (GlycoPODv2) NIH Bookshelf (Eds. Shoko Nishihara, Kiyohiko Angata, Kiyoko F. Aoki-Kinoshita, Jun Hirabayashi), JCGG, 2022.3 (#coresponding)
  5. #Manya, H., Endo, T.: Enzyme assay of protein O-mannose kinase (POMK). Glycoscience Protocols (GlycoPODv2) NIH Bookshelf (Eds. Shoko Nishihara, Kiyohiko Angata, Kiyoko F. Aoki-Kinoshita, Jun Hirabayashi), JCGG, 2022.3 (#coresponding)
  6. Imae, R., #Manya, H., Endo, T.: Enzyme assay of fukutin and fukutin-related protein (ribitol phosphate transferase, FKTN and FKRP). Glycoscience Protocols (GlycoPODv2) NIH Bookshelf (Eds. Shoko Nishihara, Kiyohiko Angata, Kiyoko F. Aoki-Kinoshita, Jun Hirabayashi), JCGG, 2022.3 (#coresponding)
  7. Akasaka-Manya, K., #Manya, H., Endo, T.: Enzyme assay of protein O-linked-mannose b1,2-N-acetylglucosaminyltransferase 1 and protein O-linked-mannose b1,4-N-acetylglucosaminyltransferase 2 (POMGNT1/2). Glycoscience Protocols (GlycoPODv2) NIH Bookshelf (Eds. Shoko Nishihara, Kiyohiko Angata, Kiyoko F. Aoki-Kinoshita, Jun Hirabayashi), JCGG, 2022.3 (#coresponding)
  8. Akasaka-Manya, K., #Manya, H., Endo, T.: Enzyme assay of protein O-mannosyltransferase (POMT1/2). Glycoscience Protocols (GlycoPODv2) NIH Bookshelf (Eds. Shoko Nishihara, Kiyohiko Angata, Kiyoko F. Aoki-Kinoshita, Jun Hirabayashi), JCGG, 2022.3 (#coresponding)
  9. Miura, N., Hanamatsu, H., Yokota, I., Akasaka-Manya, K., Manyam H., Endo, T., Shinohara, Y., Furukawa, JI.: Toolbox Accelerating Glycomics (TAG): Improving large-scale serum glycomics and refinement to identify salsa-modified and rare glycans. Int. J. Mol. Sci. 23, 13097, 2022
  10. Tamura, JI., Tamura, T., Hoshino, S., Imae, R., Kato, R., Yokono, M., Nagase, M., Ohno, S., Manabe, N., Yamaguchi, Y., Manya, H., Endo, T.: Chemical and Chemo-Enzymatic Syntheses of Glycans Containing Ribitol Phosphate Scaffolding of Matriglycan. ACS Chem. Biol. 17(6),1513-1523, 2022
  11. Tokuoka, H. Imae, R., Nakashima, H., Manya, H., Masuda, C., Hoshino, S., Kobayashi K, Lefeber, D.J., Matsumoto, R., Okada, T., Endo, T., Kanagawa, M., Toda, T.: CDP-ribitol prodrug treatment ameliorates ISPD-deficient muscular dystrophy mouse model. Nat. Commun.,13(1), 1847, 2022
  12. Imae, R., Manya, H., Endo, T.: Biosynthetic mechanisms and biological significance of glycerol phosphate-containing glycan in mammals. Molecules, 26, 6675, 2021
  13. Imae, R., #Manya, H., Tsumoto, H., Miura, Y., #Endo, T.: PCYT2 synthesizes CDP-glycerol in mammals and reduced PCYT2 enhances the expression of functionally glycosylated a-dystroglycan. J. Biochem., 170, 183-194, 2021 (#co-coresponding authors)
  14. Imae, R., Kuwabara, N., Manya, H., Tanaka, T., Tsuyuguchi, M., Mizuno, M., Endo, T., Kato, R.: The structure of POMGNT2 provides new insights into the mechanism to determine the functional O-mannosylation site on α-dystroglycan. Genes Cells, 26, 485-494, 2021
  15. Manya, H., Kuwabara, N., Kato, R., Endo, T.: FAM3B/PANDER-like carbohydrate-binding domain in a glycosyltransferase, POMGNT1. Methods Mol. Biol., 2132, 609-619, 2020
  16. Akasaka-Manya, K., Manya, H.: The role of APP O-glycosylation in Alzheimer's disease. Biomolecules, 10(11),1569, 2020
  17. Imae, R., Kuwabara, N., Manya, H., Kato, R., Endo, T.: Biosynthetic mechanisms of a unique ribitol phosphate-containing glycan by FKRP, a ribitol phosphate transferase. Trends Glycosci. Glycotech., 32(190), E195-E200, 2020
  18. Furukawa, J-I., Hanamatsu, H., Nishikaze, T., Manya, H., Miura, N., Yagi, H., Yokota, I., Akasaka-Manya, K., Endo, T., Kanagawa, M., Iwasaki, N., Tanaka, K.: Lactone-driven ester-to-amide derivatization for sialic acid linkage-specific alkylamidation. Anal Chem., 92(21), 14383-14392, 2020
  19. Akasaka-Manya, K., #Manya, H., Nadanaka, S., Kitagawa, H., Kondo, Y., Ishigami, A., #Endo, T.: Decreased ADAM17 expression in the lungs of a-Klotho reduced mouse. J. Biochem., 167(5),483-493, 2020 (#co-coresponding authors)
  20. *Kuwabara, N., *Imae, R., Manya, H., Tanaka, T., Mizuno, M., Tsumoto, H., Kanagawa, M., Kobayashi, K., Toda, T., Senda, T., #Endo, T., #Kato, R.: Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy. Nat. Commun., 11(1), 303, 2020 (*co-first authors, #co-coresponding authors)
    （プレスリリース：https://www.tmghig.jp/research/release/cms_upload/e076ae356eed1708ac9dfcb29c9bba97.pdf）
  21. Imae, R., Manya, H., Tsumoto, H., Osumi, K., Tanaka, T., Mizuno, M., Kanagawa, M., Kobayashi, K., Toda, T., Endo, T: CDP-glycerol inhibits the synthesis of the functional O-mannosyl glycan of a-dystroglycan. J. Biol. Chem., 293(31), 12186-12198, 2018
    （プレスリリース：https://www.tmghig.jp/research/release/cms_upload/19b6551f72514a48f90d7545d479e34b_2.pdf）
  22. Manya, H., Mechanisms of O-mannosyl glycan biosynthesis in mammals. Trends in Glycosci. Glycotech., 31(181), SE63 - SE64, 2019
  23. Nishihara, R., Kobayashi, K., Imae, R., Tsumoto, H., Manya, H., Mizuno, M., Kanagawa, M., Endo, T., Toda, T.: Cell endogenous activities of fukutin and FKRP coexist with the ribitol xylosyltransferase, TMEM5. Biochem. Biophys. Res. Commun., 2018, 497, 1025-1030
  24. Manya, H., Endo, T.: Glycosylation with ribitol-phosphate in mammals: New insights into the O-mannosyl glycan. Biochim. Biophys. Acta., 1861(10), 2462-2472, 2017
  25. Kanagawa, M., Manya, H.: Tatsushi Toda and Tamao Endo win 107th Japan Academy Prize. Glycobiology, 27(7), 599-600, 2017
  26. Yamashita, K. et al.: Experimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography. IUCrJ, 4, 639-647, 2017
  27. Nagae, M., Mishra, S.K., Neyazaki, M., Oi, R., Ikeda, A., Matsugaki, N., Akashi, S., Manya, H., Mizuno, M., Yagi, H., Kato, K., Senda, T., Endo, T., Nogi, T., Yamaguchi, Y.: 3D structural analysis of Protein O-Mannosyl Kinase POMK, a causative gene product of dystroglycanopathy. Genes Cells, 2017, 22, 348-359
  28. Akasaka-Manya, K., Kawamura, M., Tsumoto, H., Saitoh, Y., Tachida, Y., Kitazume, S., Hatsuta, H., Miura, Y., Hisanaga, S., Murayama, S., Hashimoto, Y., #Manya, H., #Endo, T.: Excess APP O-glycosylation by GalNAc-T6 decreases Aβ production. J. Biochem., 161(1), 99-111, 2017 (#co-coresponding authors)
  29. Manya, H., Yamaguchi, Y., Kanagawa, M., Kobayashi, K., Tajiri, M., Akasaka-Manya, K., Kawakami, H., Mizuno, M., Wada, Y., Toda, T., Endo, T.: The muscular dystrophy gene TMEM5 encodes a ribitol β1,4-xylosyltransferase required for the functional glycosylation of dystroglycan. J. Biol. Chem., 291(47), 24618-24627, 2016
    （プレスリリース：http://www.tmghig.jp/research/release/cms_upload/press20161017.pdf）
  30. Kuwabara, N., Manya, H., Yamada, T., Tateno, T., Kanagawa, M., Kobayashi, K., Akasaka-Manya, K., Hirose, Y., Mizuno, M., Ikeguchi, M., Toda, T., Hirabayashi, J., Senda, T., #Endo, T., #Kato, R.: Carbohydrate-binding domain of the POMGnT1 stem region modulates O-mannosylation sites of a-dystroglycan. Proc. Natl. Acad. Sci. USA, 113 (33), 9280-9285, 2016 (#co-coresponding authors)
    （プレスリリース：http://www.tmghig.jp/research/release/cms_upload/press20160805.pdf）
  31. *Yang, H., *Manya, H., Kobayashi, K., Jiao, H., Fu, X., Xiao, J., Li, X., Wang, J., Jiang, Y., Toda, T., Endo, T., Wu, X., Xiong, H.: Analysis of phenotype, enzyme activity and genotype of Chinese patients with POMT1 mutation. J. Hum. Genet., 61(8), 753-759, 2016 (*co-first authors)
  32. Akasaka-Manya, K., Manya, H., Endo, T.: Function and change with aging of a-klotho in the kidney. Klotho, Vitam. Horm., vol. 101 (Gerald Litwack ed.), Academic Press, pp 239-256, 2016
  33. *Kanagawa, M., *Kobayashi, K., *Tajiri, M., *Manya, H., *Kuga, A., Yamaguchi, Y., Akasaka-Manya, K., Furukawa, J., Mizuno, M., Kawakami, H., Shinohara, Y., #Wada, Y., #Endo, T., #Toda, T.: Identification of a post-translational modification with ribitol-phosphate and its defect in muscular dystrophy. Cell Rep., 14(9), 2209-2223, 2016 (*co-first / #co-coresponding authors)
    （プレスリリース：http://www.tmghig.jp/research/release/cms_upload/press20160229.pdf）
  34. Xu, M., Yamada, T., Sun, Z., Eblimit, A., Lopez, I., Wang, F., Manya, H., Xu, S., Zhao, L., Li, Y., Kimchi, A., Sharon, D., Sui, R., #Endo, T., #Koenekoop, R.K., #Chen, R. : Mutations in POMGNT1 cause non-syndromic retinitis pigmentosa. Hum. Mol. Genet., 25(8), 1479-1488, 2016 (#co-coresponding authors)
    （プレスリリース：http://www.tmghig.jp/research/release/cms_upload/press20160222.pdf）
  35. Riemersma M.,, Sean Froese, D., van Tol, W., Engelke, U.F., Kopec, J. van Scherpenzeel, M., Ashikov, A., Krojer, T., von Delft, F., Tessari, M., Buczkowska, A., Swiezewska, E., Jae, L.T., Brummelkamp, T.R., Manya, H., Endo, T., van Bokhoven, H., Yue, W.W., Lefeber, D.J. : Human ISPD is a cytidyltransferase required for dystroglycan O-mannosylation. Chem. Biol., 22(12), 1643-1652, 2015
  36. Xin, X., Akasaka-Manya, K., #Manya, H., Furukawa, J., Kuwabara, N., Okada, K., Tsumoto, H., Higashi, N., Kato, R., Shinohara, Y., Irimura, T., #Endo, T. : POMGNT1 Is Glycosylated by Mucin-Type O-Glycans. Biol. Pharm. Bull., 38(9), 1389-1394, 2015 (#co-corresponding authors)
  37. Manya, H., Endo T. : O-Mannosyl glycan and muscular dystrophy. Sugar chains (Suzuki, T., Ohtsubo, K., Taniguchi, N.), Springer, 235-238, 2015.
  38. Kizuka, Y., Kitazume, S., Fujinawa, R., Saito, T., Iwata, N., Saido, T.C., Nakano, M., Yamaguchi, Y., Hashimoto, Y., Staufenbiel, M., Hatsuta, H., Murayama, S., Manya, H., Endo, T., Taniguchi, N. : An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease. EMBO Mol. Med., 7, 175-189, 2015.
  39. Yaji, S., Manya, H., Nakagawa, N., Takematsu, H., Endo, T., Kannagi, R., Yoshihara, T., Asano, M., Oka, S. : Major glycan structure underlying expression of the Lewis X epitope in the developing brain is O-mannose-linked glycans on phosphacan/RPTPβ. Glycobiology, 25, 376-385, 2015.
  40. Manya, H., Endo T.: Protein O-linked-mannose β-1,2-N-acetyl glucosaminyltransferase 1 (POMGNT1). Handbook of Glycosyl transferases and related genes. (Taniguchi N., Honke K., Fukuda M., Narimatsu H., Yamaguchi Y., Angata T.), Springer, 409-414, 2014
  41. Manya, H., Endo T.: Protein O-mannosyl-transferase 1,2 (POMT1,2). Handbook of Glycosyltransferases and related genes. (Taniguchi N., Honke K., Fukuda M., Narimatsu H., Yamaguchi Y., Angata T.), Springer, 173-179, 2014
  42. Akasaka-Manya, K., Manya, H., Kizuka, Y., Oka, S., Endo, T. : α-Klotho mice demonstrate increased expression of the non-sulfated N-glycan form of the HNK-1 glyco-epitope in kidney tissue. J. Biochem., 156, 107-113, 2014.
  43. Tamaru, Y., Avşar-Ban, E., Manya, H., Endo, T., Akiyama, S. : Molecular characterization of protein O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 in Zebrafish. J. Glycomics Lipidomics, 4, 111, 2014.
  44. Morise, J., Kizuka, Y., Yabuno, K., Tonoyama, Y., Hashii, N., Kawasaki, N., Manya, H., Miyagoe-Suzuki, Y., Takeda, S., Endo, T., Maeda, N., Takematsu, H., Oka, S.: Structural and biochemical characterization of O-mannose-linked human natural killer-1 glycan expressed on phosphacan in developing mouse brains. Glycobiology, 24, 314-324, 2014.
  45. Jiao, H., Manya, H., Wang, S., Zhang, Y., Li, X., Xiao, J., Yang, Y., Kobayashi, K., Toda, T., Endo, T., Wu, X., Xiong, H. : Novel POMGnT1 mutations cause muscle-eye-brain disease in Chinese patients. Mol. Genet. Genomics., 288, 297-308, 2013.
  46. Ogawa, M., Nakayam, Y., Nakamura, N., Kurosaka, K., Manya, H., Kanagawa, M., Endo, T., Furukawa, K., Okajima, T. : GTDC2 modifies O-mannosylated α-dystroglycan in the endoplasmic reticulum to generate N-acetyl-glucosamine epitopes reactive with CTD110.6 antibody. Biochem. Biophys. Res. Commun., 440, 88-93, 2013.
  47. Avşar-Ban, E., Ishikawa. H., Akiyama, S., Manya, H., Endo, T., Tamaru, T. Functional and heterologous expression of human protein O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 in zebrafish. J. Biosci. Bioengineer., 114, 237-239, 2012
  48. Nakagawa, N., Manya, H., Toda, T., Endo, T., Oka, S. Human natural killer-1 sulfotransferase (HNK-1ST)-induced sulfate-transfer regulates laminin-binding glycans on α-dystroglycan. J. Biol. Chem., 287, 30823-30832, 2012
  49. Kuga, A., Kanagawa, M., Sudo, A., Chan, Y. M., Tajiri, M., Manya, H., Kikkawa, Y., Nomizu, M., Kobayashi, K., Endo, T., Qi, L. Lu, Wada, Y., and Toda, T. Absence of post-phosphoryl modification in dystroglycanopathy mouse models and wild-type tissues expressing a non-laminin binding form of α-dystroglycan. J. Biol. Chem., 287, 9560-9567, 2012.
  50. Manya, H : Biosynthetic pathway of O-mannosyl glycan in mammals. Trends Glycosci. Glycotech., 23, 272-283, 2011.
  51. Akasaka-Manya, K., Manya, H., Hayashi, M., and Endo, T. Different roles of the two components of human protein O-mannosyltransferase, POMT1 and POMT2. Biochem. Biophys. Res. Commun., 411, 721-725, 2011.
  52. Akasaka-Manya, K., Manya, H., Mizuno, M., Inazu, T., and Endo, T. Effects of length and amino acid sequence of O-mannosyl peptides on substrate specificity of protein O-linked mannose β1,2-N-acetylglucosaminyltransferase 1 (POMGnT1). Biochem. Biophys. Res. Commun., 410, 632-636, 2011.
  53. St.-Pierre, C., Manya, H., Ouellet, M., Clark, G. F., Endo, T., Tremblay, M. J., Sato, S. : Host-soluble Galectin 1 promotes HIV-1 replication through a direct interaction with glycans of viral gp120 and host CD4. J. Virol., 85, 11742-11751, 2011.
  54. Manya, H., Akasaka-Manya, K., Nakajima, A., Kawakita, M., and Endo, T. Role of N-glycans in maintaining the activity of protein O-mannosyltransferases POMT1 and POMT2. J. Biochem., 147, 337-344, 2010.
  55. Manya, H., Akasaka-Manya, K., and Endo, T. Klotho protein deficiency and aging. Geriatr. Gerontol. Int., 10(Suppl. 1), S80-S87, 2010.
  56. Akasaka-Manya, K., Manya, H., Sakurai, Y., Wojczyk, B.S., Kozutsumi, Y., Saito, Y., Taniguchi, N., Murayama, S., Spitalnik, S.L., and Endo, T. Protective effect of N-glycan bisecting GlcNAc residues on β-amyloid production in Alzheimer's disease. Glycobiology, 20, 99-106, 2010.
  57. Akasaka-Manya, K., Manya, H., Sakurai, Y., Wojczyk, B.S., Spitalnik, S.L., and Endo, T. Increased bisecting and core fucosylated N-glycans on mutant human amyloid precursor proteins. Glycoconjugate J., 25, 775-786, 2008.
  58. Manya, H., Chiba, A., Yoshida, A., Wang, X., Chiba, Y., Jigami, Y., Margolis, R. U. and Endo, T.: Demonstration of mammalian protein O-mannosyltransferase activity: Coexpression of POMT1 and POMT2 required for enzymatic activity. Proc. Natl. Acad. Sci. USA, 101(2), 500-505, 2004.
  59. Manya, H., Inomata. M., Fujimori, T., Dohmae, N., Sato, Y., Takio, K., Nabeshima, Y., Endo, T.: Klotho protein deficiency leads to overactivation of µ-calpain. J. Biol. Chem., 277(38), 35503-35508, 2002.
  60. Yoshida, A., Kobayashi, K., Manya, H., Taniguchi, K., Kano, H., Mizuno, M., Inazu, T., Mitsuhashi, H., Takahashi, S., Takeuchi, M., Herrmann, R., Straub, V., Talim, B., Voit, T., Topaloglu, H., Toda, T. and Endo, T.: Muscular Dystrophy and Neuronal Migration Disorder Caused by Mutations in a Glycosyltransferase, POMGnT1. Dev. Cell, 1(5), 717-724, 2001.

【和文】

  1. 萬谷博：哺乳類におけるO-マンノース型糖鎖の構造と生合成機構. Glycoword, Glyco forum, GD-C07J, 2024, (https://www.glycoforum.gr.jp/glycoword/indexJ.html)
  2. 赤阪-萬谷啓子、萬谷博：老化にともなうKlothoの減少と糖鎖修飾変化の意義. 腎臓内科, 18(1), 39-46, 2023.7
  3. 今江理恵子：薬学研究への道「基礎研究の医療への応用を目指して」.薬奨ニュース, 36, 8, 2023.7
  4. 萬谷博：加齢脳とカルシウム代謝異常. 老年精神医学雑誌 33(5), 501-508, 2022
  5. 赤阪啓子、萬谷博：老化モデル動物, 食品免疫学事典（日本食品免疫学会編, 朝倉書店）2021.11.1
  6. 今江理恵子, 桑原直之, 萬谷博, 加藤龍一, 遠藤玉夫, 筋ジストロフィー症原因遺伝子産物FKRPによるリビトールリン酸含有糖鎖の合成機構. 生化学, 92(6), 811-816, 2020
  7. 今江理恵子, 萬谷博：筋ジストロフィー症に関わる糖鎖が作られるしくみ. 研究トピックス, 研究所NEWS, 東京都健康長寿医療センター, 298, 4, 2020.6
    （研究所NEWS：https://www.tmghig.jp/research/publication/news/pdf/298.pdf）
  8. 萬谷博, 遠藤玉夫, 哺乳類におけるリビトールリン酸含有糖鎖の発見とO-マンノース型糖鎖の生合成機構の解明, 生化学, 89, 605-612, 2017
  9. 桑原直之, 加藤龍一, 萬谷博, 遠藤玉夫, POMGnT1の構造解析による筋ジストロフィー疾患発症機序解明, 日本結晶学会誌, 2017, 59,114-120
  10. 萬谷博: 研究トピックス「先天性筋ジストロフィー症と網膜色素変性症の原因遺伝子の発見」.研究所NEWS, 東京都健康長寿医療センター, 275, 4, 2016.7
    （研究所NEWS：http://www.tmghig.jp/research/publication/news/pdf/rj_no275.pdf）
  11. 萬谷博: 抗老化タンパク質とミネラルバランス. 百歳万歳Gabin, エヌシィシィ, 38(3), 34-35, 2016
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